Robotic Engineering: An Integrated Approach by Klafter: Free download. Robotics engineering: an integrated approach.. Richard D. Klafter | Thomas A. Chmielewski | Michael Nigen
DUBAI (Reuters) - The U.N. migration agency said on Monday it was withdrawing from Yemen because of security risks.
Hammad al-Asadi, spokesman for the United Nations High Commissioner for Refugees (UNHCR), confirmed the agency was leaving.
“Yemen’s security situation has deteriorated to the point where UNHCR is withdrawing staff in Sanaa and other areas,” he told Reuters by phone. “There is an increased danger of targeted attacks.”
Yemeni security officials said on Sunday that the United Nations, which has more than 30,000 staff in the country, had closed a number of its offices and was withdrawing non-essential personnel after Houthi rebels shot down a U.N. plane last week. two additional polymers, 4-vinylpyridine (Fig. S4) and 4-vinylpyridine-*N*-oxide (Fig. S5) that are also known to bind to DNA, were used to test the specific binding of the obtained macroions to DNA. A detection limit of 10^--9^ [m]{.smallcaps} for the selected DNA probe was found by fluorescent confocal microscopy. Owing to the high selectivity of the obtained material, it is potentially useful for monitoring the dynamic changes in the DNA level within a cell ([Fig. 9](#fig9){ref-type="fig"}).
![Confocal microscopy images of HeLa cells incubated with probe-Pt-HA-PQ at different incubation times. The intensity of the red fluorescence is related to the amount of DNA. Scale bar = 10 μm.](c9sc01463b-f9){#fig9}
Conclusions
===========
In summary, we have synthesized a new macroionic probe for monitoring DNA level in living cells. The polycationic macroion is functionalized with a DNA recognition moiety (Zn^2+^-NTA) and a quencher (BHQ), which can specifically bind to the minor groove of DNA in the presence of Zn^2+^. Due to the photoinduced electron transfer process, the emission of BHQ is suppressed under ac619d1d87
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